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KMID : 0380020130280050310
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 5 p.310 ~ p.314
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Overexpression of GFP-AFP Chimera Protein using Recombinant Escherichia coli and Analysis of Anti-freezing Characteristics
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Ko Ji-Seun
Hong Soon-Ho
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Abstract
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Antifreeze peptide from Myoxocephalus octodecemspinosus was overexpressed and purified in Escherichia coli. Green fluorescence protein-AFP chimera was constructed by integrating gfp and afp genes. Produced GFP-AFP chimera protein was purified using polyhistidine tag which was inserted at C-terminus. By addition of GFP-AFP chimera protein, freezing point of elution buffer was decreased from -13oC to -20oC. This result suggested that GFP-AFP chimera can be considered as a potential candidate of novel inhibitor for gas hydrates.
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KEYWORD
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Antifreeze peptide, Hydrate, Green fluorescence protein, Escherichia coli
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